Fluorescent reporters have been widely applied in biomedical research given their easy observation under the microscope. Our recent work by generating a dual reporter platform in hiPSCs has shown the robustness of fluorescent reporters used for delineating cardiac lineages and isolating lineage-specific cardiovascular cells. The work facilitates understanding of cardiac lineage determination during in vitro differentiation and further application of lineage-specific cardiovascular cells in precise disease modeling and drug testing. We aim to further apply different fluorescent reporter platforms coupled with advanced live-cell imaging modalities, CRISPR screening, and multi-omics techniques to 1) better understand cell fate determination which accelerates the process of directed differentiation in vitro; 2) delineate the genetic significance in the efficacy and toxicity of drugs.